Glycolysis occurs in the cytoplasm in the absence of O2and involves the following:
1. Two molecules of ATP are use to phosphorylate glucose and start glycolysis
2. The phosphorylated molecule is then broken down in a series of reactions into two three carbon molecules (lysis).
3. Two molecules of NAD+ capture and are reduced to 2 molecules of
3. Four molecules of ATP are produced by substrate phosphorylation
4. The end product pyruvate may then either undergo areobic respiration in the mitochondria or anaerobic respiration (fermentation).
Summary of Products of Glycolysis (Net Gain of 2 ATP)

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